Table 1 |
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Description of stable cell populations.a |
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| Cell lines |
Expression vectorsb |
Antibiotic selection (concentration, μg/ml) |
|
|
||
| Raji |
NAc |
NA |
| Raji/DC-SIGN |
pcDNA3-DC-SIGN |
Neomycin (500) |
| Raji/DN-CIITA |
pDN-CIITA (300–1130) |
Neomycin (500) |
| Raji/DC-SIGN/DN-CIITA |
pMX-DC-SIGN, pDN-CIITA (300–1130) |
Neomycin (500) |
| K562 |
NA |
NA |
| K562/LFA-1 |
pCDB1, pCDL1, pCMV-hph |
Hygromycin (200) |
| K562/DC-SIGN |
pcDNA3-DC-SIGN |
Neomycin (500) |
| K562/DC-SIGN/LFA-1 |
pcDNA3-DC-SIGN, pCDB1, pCDL1, pCMV-hph |
Neomycin (500) Hygromycin (200) |
| K562/WT-CIITA |
pWT-CIITA |
Neomycin (500) |
| K562/WT-CIITA/LFA-1 |
pWT-CIITA pCDB1, pCDL1, pCMV-hph |
Neomycin (500) Hygromycin (200) |
| K562/DC-SIGN/WT-CIITA |
pBABE-DC-SIGN pWT-CIITA |
Puromycin (1) Neomycin (500) |
| K562/DC-SIGN/WT-CIITA /LFA-1 |
pMX-DC-SIGN, pWT-CIITA, pCDB1, pCDL1, pCMV-hph |
Neomycin (500) Hygromycin (200) |
|
|
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a Stable cell lines were transduced with murine leukemia virus (MLV) vectors [44] or stably transfected with other constructs. After vector transduction or selection for antibiotic resistance, cells were subjected to FACS to enrich desired populations. b Construct information is provided in the Methods section. c NA, not applicable. |
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Wu et al. Retrovirology 2004 1:14 doi:10.1186/1742-4690-1-14 |
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