Table 2

Expression of HIV-1 receptors and adhesion molecules on donor cells.^a

^a Proteins expressed on the surface of the donor cells were stained and analyzed by FACS. Isotype-matched mouse IgG controls were also examined. Cell population expression levels indicated as ++, +, and +/- demonstrated a mean fluorescence of antibody staining greater than 50, 10, and 7.5, respectively. No antibody and isotype control stainings typically displayed a mean fluorescence intensity of 5 in the FL-2 channel, using a Becton-Dickinson FACSCalibur instrument. ND, not done. Staining of parental Raji and K562 cells was uniformly negative for DC-SIGN and showed a similar expression pattern of these ligands (data not shown). ^b Immature monocyte-derived dendritic cells were stained at day 7 of culture in the presence of cytokines [5]. ^c HLA-DP and -DQ expression was also examined and results were similar to what is shown for HLA-DR.

Wu et al. Retrovirology 2004 1:14   doi:10.1186/1742-4690-1-14