Figure 6.

Inhibitory effects of Protegrin-1 and LL37 on HIV-1 Env mediated vector entry (A, B) and HIV-1 infection (C,D). A lentiviral vector transferring the GFP gene (VGΔBH-SIN) was incubated at increasing concentrations of LL37 (A) or Protegrin-1 (B) prior to transduction of P4CCR5 cells. The vector titer is given as percentage of the titer of the vector incubated in the absence of HDPs. Wild type HIV-1 was incubated with increasing concentrations of LL37 (C) and Protegrin-1 (D). The virus titer was subsequently determined on P4CCR5 indicator cells and is expressed as percentage of the titer of the untreated HIV-1 virus stock. The toxicity of LL37 and Protegrin-1 was determined in parallel using the BrdU incorporation assay.