Figure 1.

Toxin B inhibits VLP release. A. Toxin B dose dependent inhibition of VLP production. Supernatants of MVA infected/HIV-1 Gag transfected HeLa-CD4 cells, treated or not with various concentrations of Toxin B for 16 h, were clarified by low speed centrifugation and treated or not (no) with Trypsin (ty), Triton X-100 (tx) or with Triton X-100 and Trypsin (tt). VLPs were recovered by centrifugation and subjected to Western Blot analysis. B. Lysates of cells from panel A were subjected to Western Blot analysis. (mock: cells transfected with the vector without any insert, T+: is a cellular extract of HIV-1_NDK infected H9 cells). C. p24 antigen was quantified in lysates from panel A by ELISA.