Figure 3.

Engagement of small GTP binding proteins in HIV-1 release. Jurkat HIV-1 infected cells were incubated for 20 h with various concentrations of bacterial toxins and Cyto D. A) Clarified supernatants of the culture medium were harvested for p24 quantification by ELISA. Vertical axis indicates the relative HIV-1 production expressed as a percentage of the p24 antigen in the absence of toxin treatment. B) Titres of infectious virus (TCID₅₀) released/pg of p24 from the highest toxin concentration dose from infected cells shown in panel A. Data presented corresponds to one out of three independent experiments. Each experiment was performed in triplicate.