Figure 4.

Toxin treatment does not affect intracellular HIV-1 Gag assembly. Non denaturing cytoplasmic lysates of HIV-1_NDK Jurkat infected cells treated or not with 0.5 μg/ml of Toxin B, LT, or Cyto D for 16 h, were centrifuged through a discontinuous sucrose gradient. Eleven fractions were collected from top to bottom, concentrated by high speed centrifugation, and analysed by Western Blot. Vertical axis shows the sucrose density fractionation in g/ml. Arrows indicate Pr55gag migration.