Figure 4.

No inhibition of third generation lentiviral vectors by Tat-P was observed. To test that the Tat-P inhibition activity is specifically targeting HIV TAT protein, 293T cells were cotransfected with four plasmids of a third generation lentiviral vector system that is independent of the TAT protein. Then, 200 μM of Tat-P, Con-P1, and Con-P2 peptides, or a PBS control were added to the 293T cells for 6 hours. The supernatants containing the viral particles were collected and added to freshly cultured 293T cells to measure viral titers.