Table 1

List of primers used for PCR amplification

Target region

Primer name (sequence 5'-3')

LTR

1R

F: LTR1 (cacacaaggctayttcctga)

R: JL17 (cattctgcagcttcctcattgat)

2R

F: LTR3 (tggatggtgctwcaagytagt)

R: LTRrev (tgctagagattttccacactgac)

tat 1st exon

1R

F: polseq2 (cgggtttattacagggacagc)

R: ES33 (cattgccactgtcttctgctc)

2R

F: TECORI (cagaataggaattctgcgacagagaag) ^*1

R: TE1 (gggatatgggttgctttgatagagaagc)

tat 2nd exon

1R

F: KS2mod (ttctatagtggatagagttaggaaggg)

R: OFM19mod (gcactcaaggcaagctttattgaggc)

2R

F: TE2 (gcttctctatcaaagcaacccatatccc)

R: TXHOI (acaggctcctcgaggtcgtccc) ^*2

β-actin

F: β1 (ggacctgactgactacctcatgaa)

R: β2 (gatccacatctgctggaaggtgg)

1R = 1st round, 2R = 2nd round. Introduced EcoRI (^*1) and XhoI (^*2) restriction sites are underlined. F: Forward. R: Reverse.

Turk et al. Retrovirology 2006 3:14   doi:10.1186/1742-4690-3-14