Detection of the HTLV-I antisense transcript in HTLV-I-infected cell lines. (A) Positioning of the HBZ antisense ORF in the HTLV-I proviral DNA. Primers used for RT-PCR experiments and the expected size of the amplified signal are indicated above the enlarged HBZ ORF. (B) RT-PCR analyses were performed on RNA samples from HTLV-I-infected cell lines using the 21-5 primer for RT and primer combinations presented in A for PCR analysis. Samples were tested for DNA contamination in RNA samples (lanes 1–2; no RT and no RT primer) and autopriming (lanes 3–4; in the presence of RT with no added RT primer). CTL represents PCR analysis with no added cDNA or RNA. M = 100 bp marker (the asterisk indicates the 600 bp band). Lanes 5 and 6 show the results of PCR using primers 23-3/21-5 and 21-4/21-5 to generate products of 400 bp and 450 bp, respectively.
Cavanagh et al. Retrovirology 2006 3:15 doi:10.1186/1742-4690-3-15