Detection of the HTLV-1 antisense transcript in HTLV-I-producing 293T cells. (A) K30 and K30-3'/5681 proviral DNA constructs are depicted. The deleted region for the latter construct is shown. (B-C) 293T cells were transfected with 5 μg K30 (B) or K30-3'/5681 (C). RT-PCR analyses was then conducted on RNA isotated from transfected 293T cells. RT-PCR conditions and controls were performed as in fig. 1. M = lambda DNA (EcoRI/HindIII) marker.
Cavanagh et al. Retrovirology 2006 3:15 doi:10.1186/1742-4690-3-15