Functional properties of the SP1-derived HBZ isoform. (A) 293T cells were co-transfected with 2 μg of K30-LUC and increasing concentrations of pcDNA3.1-Myc-His HBZ SP1 Δ 5'UTR, along with the β-gal reporter vector. (B) CEM cells were co-transfected with the collagenase promoter-driven luciferase reporter construct (2 μg), pcDNA-c-Jun (1 μg), pcDNA3.1-Myc-His HBZ SP1 Δ 5'UTR (2 and 5 μg), and the β-gal reporter vector (5 μg). Luciferase activities represent the mean value of three measured samples ± S.D and are expressed as normalised RLU for 5 × 106 cells. Fold inductions in panel B were calculated with respect to CEM cells transfected in the absence of pcDNA-c-Jun (set at a value of 1).
Cavanagh et al. Retrovirology 2006 3:15 doi:10.1186/1742-4690-3-15