Figure 1.

Ultrastructural subcellular localization of HIV-1 derived vector genomes including the central DNA Flap (Flap +). Electron micrographs showing MT4 cells 48 hr following transduction with the TRIP Flap+ vector. Vector DNA genomes including the DNA Flap are found predominantly within the nucleus. N = nucleus; ne = nuclear envelope; nu = nucleolus; C = cytoplasm. Images show one low and four high magnification micrographs. The first high magnification micrograph is an enlargement from the low magnification image. The other three are taken from other independent experiments. All are highly representative of the data obtained. Arrows point to clusters of immunogold labeled vector DNA.