Table 3 |
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|
Definition of HIV-2 Protease susceptibility to LPV and SQV, from infected individuals |
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| Phenotype (transformed yeast) (% cell growth) |
IC50 [μM] |
IC50isolate/IC50ROD |
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|
|
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| Protease |
LPV |
SQV |
LPV |
SQV |
LPV |
SQV |
| ROD |
91.5+/-1.2 |
75.0+/-7.0 |
16.6+/-2.5 |
149.7+/- 7.2 |
1.00 |
1.00 |
| MRT-1 |
50.0+/-3.0 |
71.0+/-5 |
48.0+/-3.2 |
200.1+/-5.1 |
2.89 |
1.34 |
| MRT-7 |
100.0+/-4.7 |
98.0+/-6 |
10.8+/-5.0 |
68.9+/-6.2 |
0.72 |
0.46 |
| MRT-8 |
82.0+/-7.5 |
55.0+/-6.3 |
12.5+/-1.5 |
553.9+/-4.2 |
0.66 |
4.23 |
| MRT-20 |
71.5+/-1.2 |
78.0+/-3.0 |
23.2 +/-4.4 |
118.0+/-6.3 |
1.40 |
0.79 |
| MRT-22 |
98.5+/-1.0 |
82.0+/-7.1 |
15.1+/-4.3 |
104.8+/-7.1 |
0.91 |
0.70 |
| MRT-25 |
100.0+/-2.0 |
100.0+/-9.4 |
19.9+/-4.6 |
48.5+/-4.7 |
1.20 |
0.03 |
| MRT-29 |
93.0+/-2.0 |
72.0+/-3.0 |
13.9+/-5.5 |
153.1+/-3.0 |
0.84 |
1.02 |
|
|
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|
Viral Protease genes from HIV-2 infected individuals, as well as from the ROD isolate, were PCR amplified, sub-cloned in pRS316Gal1/10 expression vector and used to transform yeast cells. Protease susceptibility to LPV and SQV was determined by defining the % of cell growth in galactose in the presence of inhibitors (as in table 1), and IC50 values were defined as in Fig 2A. |
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|
M'Barek et al. Retrovirology 2006 3:58 doi:10.1186/1742-4690-3-58 |
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