Figure 4.

Dox regulated replication of the mini-rtTA virus rtTAΔ6^A. SupT1 cells were infected with rtTAΔ6^A virus (1 ng CA-p24). The culture was split and the cells were cultured with dox (upper panels) or without (lower panels). Virus replication was monitored by CA-p24 Elisa on the culture supernatant. At day 9 post infection, both cultures were washed and each culture split into one culture with dox (left panels) and one without (right panels). Filled triangles indicate cultures without dox and open triangles indicate cultures with 1000 ng/mL dox.