Figure 5.

Replication and cell killing capacity of dox inducible viruses in PBMC. (Left) Virus replication of LAI (△), HIV-rtTA (▲), rtTAΔ6^A (○), rtTAΔ6^B (◆) and rtTA without dox (×) was determined by monitoring the supernatant CA-p24 concentration after virus infection (40 ng CA-p24) in a 5 mL PBMC culture. (Right) The CD4+ and CD8+ cell populations in the infected cultures and an uninfected control culture were quantified by a 30 sec time limit FACS analysis and the CD4/CD8 ratio was calculated. The figure shows the CD4/CD8 ratio in the infections normalized for the control uninfected PBMC culture.