Figure 3.

CypA/CA interaction in FIV WT and mutants. (A) The CA amino acid sequence alignment of FIV with HIV-1. The proline to alanine and the arginine to alanine mutations are in bold, and gaps are indicated with "-". (B) Infectivity of FIV wild type and mutants under the TrimCyp restriction. FIVs wild type and mutants were transduced to CRFK or CRFK-TrimCyp cells to test their sensitivity to TrimCyp restriction. The % GFP determined by flow cytometry is the readout of the infectivity. (C) GST-CypA pull-down of CA from wild type and mutant FIV. The upper panel shows 10% of input FIV virions used for the GST-CypA pull-down assay, and the lower panel shows the CA pulled down by GST-CypA. FIV virions are detected by anti-FIV CA antibody.