Figure 1.

Human cell engraftment in the peripheral blood of CD34 cell-reconstituted Rag2^-/-γc^-/- mice and duration of engraftment. Conditioned neonatal mice were injected with CD34 cells intrahepatically. At different times post-reconstitution, mice were bled to detect human cell engraftment. Peripheral blood cells were stained with different antibodies after RBC lysis and analyzed by FACS. (A) Cells stained with antibodies against the human panleukocyte marker CD45 at 12 weeks post-engraftment. (B) Cells stained with antibodies against the T cell markers CD3 and CD4. (C) Cells stained with antibodies against the monocyte markers CD14 and CCR5. To analyze the duration of engraftment, peripheral blood cells from an engrafted mouse were stained with antibodies against CD45 at 12 weeks (D) and 52 weeks (E) post-engraftment.