Figure 3.

Ex vivo productive HIV-1 infection in human cells differentiated in reconstituted Rag2^-/-γc^-/- mice. Thymus, spleen and lymph node tissues were collected at 16 weeks post-engraftment. Single cell suspensions were made and stimulated for 3 days with PHA and IL-2, and later challenged with HIV-1 NL4-3 HSA reporter virus. To detect productive viral infection, culture supernatants were analyzed by p24 ELISA at different days post-infection.