Table 1

Detection and quantification of HIV-1 in peripheral blood by PCR
Experiment 1




Mouse
 Infection
 1 w
 11 w
 24 w
 30 w
17
 Ctrl
 -
 -
 -
 -
40
 Ctrl
 -
 -
 -
 -
1
 N+G
 +
 n/a
 n/a
 n/a
2
 N+G
 +
 n/a
 n/a
 n/a
9
 N+G
 + (369,000)a
 +
 +
 + (31,710)b
16
 N+G
 + (165,000)a
 +
 + (12,200,000)a
 + (81,440)b
21
 G
 +
 +
 +
 + (8,850)b
24
 G
 +
 +
 +
 + (17,220)b
Experiment 2




Mouse
 Infection
 1 w
 9 w
 14 w
 20 w
50
 Ctrl
 -
 -
 -
 -
51
 Ctrl
 -
 -
 -
 -
63
 N
 +
 n/a
 n/a
 n/a
64
 N
 +
 + (28,000)a
 n/a
 n/a
65
 N
 +
 n/a
 n/a
 n/a
70
 N
 +
 n/a
 n/a
 n/a
56
 N+G
 + (253,000)a
 +
 + (52,250)a
 n/a
71
 B
 +
 + (436,000)a
 +
 + (261,140)b
72
 B
 +
 + (62,300)a
 +
 + (480,770)b
75
 B
 +
 + (65,230)b
 n/a
 n/a
76
 B
 +
 + (430,280)b
 n/a
 n/a
88
 B
 +
 + (1,305,200)b
 n/a
 n/a

DNA PCR and RT-PCR analyses were performed on peripheral blood cells and plasma, respectively from infected RAG-hu mice using primers specific to the HIV-1 pol gene. Virus detection by one or both assays at various weeks post-infection is depicted as (+), while a lack of detection by both assays is depicted as such (-). Key: Ctrl = uninfected, N = NL4-3, G = NLENG1-IRES, B = BaL, n/a = available (sacrificed), a = viral load determined by Amplicor method, b = viral load determined with primers towards the LTR. Quantitative RT-PCR was performed on select samples to determine viral load per milliliter of plasma, as indicated in parentheses. Viral loads were initially determined with the Amplicor test (Roche Diagnostics). Later samples were analyzed with another primer set towards the HIV-1 LTR [41].

Berges et al. Retrovirology 2006 3:76   doi:10.1186/1742-4690-3-76

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