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Phosphorylation of HIV-1 Tat by CDK2 in HIV-1 transcription

Tatyana Ammosova1 email, Reem Berro4 email, Marina Jerebtsova5 email, Angela Jackson2 email, Sharroya Charles3 email, Zachary Klase4 email, William Southerland2 email, Victor R Gordeuk1 email, Fatah Kashanchi4 email and Sergei Nekhai1,2,4 email

Center for Sickle Cell Disease, Howard University College of Medicine, 520 W Street N.W., Washington, DC 20059, USA

Department of Biochemistry and Molecular Biology, Howard University College of Medicine, 520 W Street N.W., Washington, DC 20059, USA

Program in Genetics, Howard University College of Medicine, 520 W Street N.W., Washington, DC 20059, USA

Department of Biochemistry and Molecular Biology, The George Washington University Medical Center, 2300 I Street N.W., Washington, DC 20037, USA

Children's National Medical Center, CRI Center III, 111 Michigan Ave., N.W. Washington, D.C. 20010-2970, USA

author email corresponding author email

Retrovirology 2006, 3:78doi:10.1186/1742-4690-3-78

Published: 3 November 2006

Additional files

Additional File 1:

Purification of CDK2/cyclin E. Mixed mono Q fractions of CDK2 and cyclin E (Mono Q lane) were purified on Superdex column. Fractions 37, 39, 41, 43, and 45 were analyzed for the presence of CDK2 and cyclin E by Coumassie staining.

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