Figure 4.

Pantropic restriction in CD4 T cells from subject W276 affects the replication of several retroviruses. A. Early reverse transcripts (RU5) analyzed by real-time PCR at various times after HIV-VSVG pseudotype challenge of CD4 T cells from subject W276 (open circles) and a representative control (filled triangles). B. Infection of CD4 T cells from subject W276 (open circles) and a control (filled triangles) with increasing amounts of HIV-VSVG pseudotype. Results (mean of three independent infections) are expressed as luciferase activity per second in cell lysates three days post-challenge. The control is representative of cells from three different controls. Error bars represent the standard deviation. C. Relative infection of CD4 T cells from subject W276 (white bars) by HIV-1, SIVmac and SIVagm particles pseudotyped with the VSVG fusion protein (n = 3, mean ± SD). Luciferase activity in cell lysates from a representative control (black bars) was attributed a value of 100%.