Modification of the Tet-On regulatory system prevents the conditional-live HIV-1 variant from losing doxycycline-control
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* Corresponding author: Atze T Das a.t.das@amc.uva.nl
Retrovirology 2006, 3:82 doi:10.1186/1742-4690-3-82
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Differential effect of CLK SR Kinases on HIV-1 gene expression: potential novel targets for therapy Raymond Wong, Ahalya Balachandran, Annie YQ Mao, Wendy Dobson, Scott Gray-Owen, Alan Cochrane Retrovirology 2011, 8:47 (17 June 2011) Despite their high homology, increasing individual CLK expression had distinct effects on HIV-1, CLK1 enhancing Gag production while CLK2 inhibited the virus. Parallel studies on the anti-HIV-1 activity of CLK inhibitors revealed a similar discrepant effect on HIV-1 expression. TG003, an inhibitor of CLK1, 2 and 4, had no effect on viral Gag synthesis while chlorhexidine, a CLK2, 3 and 4 inhibitor, blocked virus production. Chorhexidine treatment altered viral RNA processing, decreasing levels of unspliced and single spliced viral RNAs, and reduced Rev accumulation. Subsequent experiments in the context of HIV-1 replication in PBMCs confirmed the capacity of chlorhexidine to suppress virus replication.
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Research
Optimization of the doxycycline-dependent simian immunodeficiency virus through in vitro evolution Atze T Das, Bep Klaver, Mireille Centlivre, Alex Harwig, Marcel Ooms, Mark Page, Neil Almond, Fang Yuan, Mike Piatak, Jeffrey D Lifson, Ben Berkhout Retrovirology 2008, 5:44 (5 June 2008) |
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Research article
Improved single-chain transactivators of the Tet-On gene expression system Xue Zhou, Jori Symons, Rieuwert Hoppes, Christel Krueger, Christian Berens, Wolfgang Hillen, Ben Berkhout, Atze T Das BMC Biotechnology 2007, 7:6 (19 January 2007) |