Table 1

Primers used to amplify regions of NF90ctv by PCR for cloning into the pCI-neo vector.

Amplified region

Construct

5'-primer: 5'GGGGGGGAATTCGCTAGCCGCCACCAATGCAGAAGACGGAGCACATGAC3'

3'-primer: 5'GGGGGGCCCGGGGCGGCCGACTAGATGGTCAGGGACAATGGACGCTCTTT3'

NES

pCI-neo/NES NF90

5'-primer: 5'GGGGGGGAATTCGCTAGCCGCCACCAATGAATGCCCTGATGCGGTTGAA3'

3'-primer: 5'GGGGGGCCCGGGGCGGCCGACTAAGCACCCGTCGGCAAGCCCATGTCCTG3'

DRBD 1

pCI-neo/DRBD 1 NF90

5'-primer: 5'GGGGGGGAATTCGCTAGCCGCCACCAATGAAGAACCCAGTCATGGAGCTG3'

3-primer: 5'GGGGGGCCCGGGGCGGCCGACTAAGGGAAAAGTTTTTCTAGGGCAGCAAG3'

DRBD 2

pCI-neo/DRBD 2 NF90

5'-primer: 5'GGGGGGGTCGACCGCCACCAATGAAGCTTTTCCCTGACACCCCTCTCGCC3'

3'-primer: 5'GGGGGGCCCGGGCGGCCGCCTATGTAGCCTCCATGGTTGGCGCCACCAAA3'

RG-

pCI-neo/RG- NF90

Urcuqui-Inchima et al. Retrovirology 2006 3:83   doi:10.1186/1742-4690-3-83