Figure 5.

PRMT6 diminishes Rev mediated viral RNA export. HeLa cells stably transfected with mock siRNA (m, lanes 1–10) or siRNA against PRMT6 (P6si, lanes 11–16) were co-transfected with pT-REx-DEST30-HRev (lanes 2,4,6,8,10,11,13,15), pDM128 (CAT located in intron, lanes 1–16) and various amounts of myc-tagged PRMT6 (wild-type lanes 7–14, mutant lanes 3–6). At 48 hours post-transfection, CAT assays were exposed (upper panel) and fold activations calculated (lower panel) as described in 4C. The migratory positions are indicated by arrows. Similar results were observed in each of three separate assays.