Figure 5.

Inhibition of intracellular HIV-1 production in the presence of antisense LNA and LNAzymes. One hundred nanograms of HIV-1 genomic LAI plasmid was co-transfected with a renilla luciferase expression construct and the indicated amounts of antisense LNA or LNAzyme, and the HIV-1 production was measured by CA-p24 ELISA 72 hours later. (A) HIV-1 production in the presence 20 nM of the four different HIV-1 specific LNAs and 2 LNA controls (Mock 1 and Mock 2). (B) Measuring HIV-1 production in the presence of low range concentration of LNA_PBSD (0.16–20 nM). The inhibition is calculated as the average value of two independent experiments where the relative CA-p24 expression is normalized for unspecific inhibition of renilla expression. (C) Comparing the inhibitory capacity of DNA versus LNA containing antisense or 10–23 enzymes targeted to the DIS and PBSD regions. The identity and the concentration of the oligonucleotide are indicated below. The assay was performed in duplicates.