Figure 5.

Surface-engineered HIV-based particle dependent inhibition of HIV-1 replication. Panel A: Lymphocytotropic HIV-1 MN p24 antigen expression in PHA-stimulated PBLs. Panel B: Monocytotropic HIV-1 Ba-L p24 antigen expression in PHA-stimulated PBLs. Donor-J cells were PHA-treated and exposed to either no particles (filled diamonds), non-engineered HIV-based particles (open squares), B7+antiCD3 surface-engineered HIV-based particles (open circles), or B7 surface-engineered HIV-based particles (open triangles). At day 3, cultures are washed and infectious HIV-1 is added – HIV-MN in Panel A and HIV-Ba-L in Panel B. Aliquots are removed at 3, 7, 12, and 17 days and HIV-1 encoded p24 antigen expression is determined by ELISA. Particle preparations used in this figure were PEG-concentrated and inactivated to render them non-infectious.