Figure 3.

Prolonged passage of HIV-1 in the presence of CQ. An R5X4 virus (293.10) was cultured for 206 days in the presence or absence of CQ (100 μM). The concentration of CA-p24 was determined in culture supernatants on either day 7 or 10 of culture and 15 ng/ml CA-p24 was added to fresh CD4⁺ T-lymphocytes. The culture was monitored for CA-p24 and the culture in the absence of CQ is depicted with a solid line and the culture in the presence of CQ is depicted as a broken line.