Short report
Centrosomal pre-integration latency of HIV-1 in quiescent cells
-
* Corresponding author: Ali Saïb ali.saib@paris7.jussieu.fr
1 CNRS UMR7151, Université Paris 7, Hôpital Saint-Louis, Paris, France
2 INSERM ERI 19, Université François Rabelais & CHRU, Tours, France
3 INSERM U567, CNRS UPR8104, Institut Cochin, Paris, France
4 INSERM U662, Laboratoire d'Immunologie et d'Histocompatibilité AP-HP, Paris, France
Retrovirology 2007, 4:63 doi:10.1186/1742-4690-4-63
Published: 10 September 2007Abstract
Human immunodeficiency virus type 1 (HIV-1) efficiently replicates in dividing and non-dividing cells. However, HIV-1 infection is blocked at an early post-entry step in quiescent CD4+ T cells in vitro. The molecular basis of this restriction is still poorly understood. Here, we show that in quiescent cells, incoming HIV-1 sub-viral complexes concentrate and stably reside at the centrosome for several weeks. Upon cell activation, viral replication resumes leading to viral gene expression. Thus, HIV-1 can persist in quiescent cells as a stable, centrosome-associated, pre-integration intermediate.