Research
Human immunodeficiency virus integrase inhibitors efficiently suppress feline immunodeficiency virus replication in vitro and provide a rationale to redesign antiretroviral treatment for feline AIDS
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* Corresponding author: Andrea Savarino andrea.savarino@iss.it
1 Dept. of Infectious, Parasitic and Immune-mediated Diseases, Istituto Superiore di Sanità , Viale Regina Elena, 299, 00161, Rome, Italy
2 Dept. of Experimental Pathology, Univ. of Pisa, Via San Zeno 37, 56127 Pisa, Italy
3 Pharmaco-chemical Dept., Univ. of Messina, Viale Annunziata, 98168 Messina, Italy
Retrovirology 2007, 4:79 doi:10.1186/1742-4690-4-79
Published: 30 October 2007Additional files
Additional file 1:
Ramachandran plot for the homology-based model of FIV integrase catalytic core domain. The output of an analysis conducted using MolProbity (see Ref. [41]) is shown.
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Additional file 2:
Sensitivity and reproducibility of the real-time quantitative assay and melting curve profile of specific amplicons. The text describes the experiments devised for validation of the real-time PCR assays adopted in the present study.
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Additional file 3:
Real-time quantitative assay. Sensitivity and reproducibility of the test (Panel A) and melting curve profile (Panel B). Panel A: Graphical representation of the DNA standard curve (ranging from 107 to 102 copies per reaction) based on the recombinant plasmid pGEM-T easy vector carrying the specific 159 bp integrase core fragment. The corresponding intra- and inter-assay calculations were done on the basis of the threshold cycles plotted against the logarithm of the copy numbers. The coefficient of variation and the test efficiency were calculated for each point of the standard curve. Panel B: Melting point analysis [fluorescence versus temperature (-dF1/dT)] and differentiation between the 159 bp integrase fragment and the 173 bp DNA circle amplicon. The box shows the gel analysis of amplicons.
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