Figure 4.

Effect of cGMP analogues on pAD8 replication in monocyte derived macrophages (MDM's). MDM's were cultured in RPMI medium containing 10% human serum, infected 5-7 days post culture with HIV-1 AD8 virus, and cells were treated at time of infection with cGMP agonists -8-pCPT-cGMP and Sp-8-pCPT-cGMP or cGMP antagonist (Rp-8-pCPT-cGMPs). Culture supernatants were collected every third day and assayed for virus production by RT assays. cGMP agonists increased virus production from AD8 infected MDM's while cGMP antagonists suppressed virus production.