Figure 4.

Analysis of efficiency of SIV replication in dividing vs non-dividing CEMx174 cells. CEMx174 cells (2 × 10⁶) treated or untreated with aphidicolin were inoculated by SIV with full length or truncated Env with similar titer; the amounts of input virus was determined based on the infectious index (IU/ng) as described in Methods. At 24 h after infection samples of nuclear DNA were tested for the presence of SIV DNA by real-time PCR in a TaqMan thermal cycler. Nuclear DNA samples corresponding to equal numbers of cells infected by SIV were analyzed in parallel. Fluorescence was recorded as a function of PCR amplification cycle. Quantitative SIV determinations were made by comparison with a standard curve produced by using serial dilution of plasmid DNA. The ratios of replication levels in dividing:non-dividing cells are shown.