Table 2 |
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|
Production of Gag antigen SIV in dividing and non-dividing CEMx174 cells. |
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| Virus |
MTT1 +aphid1day/+aphid 3 days (OD) |
Viability index (fold difference) |
p27 ng/ml2 -aphid.3 days |
p27 ng/ml2 +aphid.3 days (x3)3 |
|
|
||||
| SIVmac239 |
0.327/0.106 |
3 |
28 |
17 |
| SIVmac239T |
0.302/0.105 |
2.9 |
32 |
41 |
| SIVmac18 |
0.334/0.104 |
3.2 |
10 |
12 |
| SIVmac18T |
0.386/0.117 |
3.7 |
19 |
41 |
|
|
||||
|
1MTT assay is described in Methods 2Amount of Gag p27 antigen in supernatants determined by ELISA described in Methods 3amount was adjusted in according to results of MTT assay. |
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|
Vzorov et al. Retrovirology 2007 4:94 doi:10.1186/1742-4690-4-94 |
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