Figure 10.

RT-PCR analysis of inducible proinflammatory cytokine genes expressed in R1A, P8A and ICR-A cell lines. (A) Proinflammatory cytokine genes, TNF-α, TNF-β, IL-1α, IL-1β and IL-6 plus the anti-viral cytokine gene, IFN-γ, were assessed in each cell line. β-actin was used for analysis of cell protein concentration. 100 bp M, 100 bp DNA ladder marker. (B) Quantitative analysis of proinflammatory cytokine genes and the gene for IFN-γ. Expression levels were measured by densitometry. Expression of the housekeeping gene, β-actin, was measured in each preparation. *statistically significant difference (p < 0.05). §Comparisons not showing a statistically significant difference.