Figure 2.

HIV-1 expression reduces PTEN levels in primary human macrophages. (A) Images of primary macrophages transduced with HIV vector expressing HIV-1 proteins and EGFP (+) or heat-inactivated vector (-). Levels of PTEN protein in YU-2 infected macrophage (B) and HIV vector-transduced macrophages (C) as determined by Western blotting. Ratios of PTEN normalized by β-tubulin levels are shown. (D) Reverse transcriptase PCR analysis of PTEN mRNA levels following transduction of macrophage with HIV vector (+) or treatment with heat-inactivated vector (-). M: 100 bp size marker. β-tubulin and β-Actin were used for loading controls in the Western analysis and RT-PCR, respectively.