Figure 5.

Cellular hsa-mir-29a inhibits HIV-1 replication. (A) LNA-modified anti-29a affected the levels of endogenous hsa-mir-29a. LNA-modified anti-29a was co-transfected with pNL4.3 vector. Real time TaqMan microRNA quantification was performed for miR29a. hsa-mir-92 was used as an endogenous control. (B) LNA-modified anti-29a increased HIV-1 replication relative to mock LNA. p24 antigen ELISA was carried out as described previously. Data represent mean ± SEM from two independent experiments performed in triplicates.