Figure 5.

Localization and characterization of HIV-1 RNA positive cells in the human prostate infected ex vivo. Localization of HIV RNA+ cells (black silver grains) in prostate explants exposed for 17 days to R5_SF162 (A, C) or R5X4_92US723 (B) using in situ hybridization for HIV-1 gag. Combined ISH with immunostaining for cell markers was used to assess co-localization of HIV RNA+ cells with either CD68 (A) or CD3 (B, C). A'-C' correspond to higher magnification of A-C showing cells co-labelled for HIV RNA (black silver grains) and cell marker (brown cells). Scale bars = 20 μm.