Figure 2.

Clonality of BLV-infected cells over time in animals 4535 (A), 4536 (B), 4537 (C), and 4538 (D). Each sample was analyzed in quadruplicate by IPCR as detailed in the Material and Methods section. Each signal on the gel represents a cluster of BLV integration sites having the same length and therefore belonging to the same cellular clone. The absolute detection threshold of the technique was ~21 copies/150,000 PBMCs while samples harboring 1, 2, 3, and 4 signals after quadruplicate experiments corresponded to a BLV clonal frequency of 25 to 62.5, 62.5 to 1200, 1200 to 2400, and > 2400 infected cells per 150,000 PBMCs [4].