Figure 5.

Effect of HIV-1 infection on splicing factor mRNAexpression in MDM. mRNA was extracted from uninfected and HIV-infected MDM from individual donors (distinct from those used in Figs. 3 and 4) at weekly intervals over a 5 week period. RT-PCR for splicing factor message levels was performed following standardisation for GAPDH mRNA. Expression of splicing factor mRNA in infected MDM is given as a percentage of that in matched uninfected cells at each time point. Results for the representative hnRNPs A2 and B1 and the SR protein SC35 are shown (mean + SEM of 3 donors). Also shown is the relative expression normalised to mRNA levels at 7 days post-infection (bottom right hand panel).