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P24 production in HIV-1 persistently-infected cell lines exposed to TNF-α or PMA |
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| Cell line and treatment |
0 h |
24 h |
48 h |
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| J1.1 Cells |
14.07 ± 0.01 |
14.14 ± 0.05 |
18.26 ± 0.80 |
| J1.1 Cells + TNF α |
14.08 ± 0.01 |
12.86 ± 0.90 |
152.04 ± 1.50 |
| U1 Cells |
1.06 ± 0.03 |
1.60 ± 0.03 |
4.18 ± 1.11 |
| U1 Cells + PMA |
1.05 ± 0.03 |
51.96 ± 9.20 |
191.76 ± 0.48 |
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HIV-1 persistently-infected lymphoid J1.1 and monocytic U1 cells were treated with TNF-α and PMA respectively in order to stimulate viral replication. P24 antigen was determined at 0, 24 and 48 h in cell supernatants by ELISA (HIVAG-1 Monoclonal, Abbot Laboratories). Values correspond to p24 antigen per 200,000 cells, expressed in J1.1 as pg/ml and in U1 Cells as ng/ml. | |||
Fernández Larrosa et al. Retrovirology 2008 5:19 doi:10.1186/1742-4690-5-19 |
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