Figure 1.

Replication competition experiments with E40F site-directed mutants. Replication competition experiments were performed in SupT1 cells in at least two independent experiments. After four days and after 2, 4 and 6 passages the relative presence of both viruses in the culture was determined by sequencing. Shown are two representative experiments. The variability in each independent experiment is indicated by ± standard error of the mean (SEM). A: M41L+T215Y versus M41L+T215Y+E40F B: Pat A (E40F, M41L, K43E, M184V, L210W, T215Y and K219T) versus Pat A-WT40 (M41L, K43E, M184V, L210W, T215Y and K219T).