Table 3 |
|||||||||||
|
Zidovudine susceptibility analysis |
|||||||||||
| Resistance-associated amino acid in RT |
40 |
41 |
43 |
184 |
210 |
215 |
219 |
Average |
Fold |
||
| Consensus B |
E |
M |
K |
M |
L |
T |
K |
IC50 (nM) |
Increase |
||
|
|
|||||||||||
| Wild type (HXB2) |
. |
. |
. |
. |
. |
. |
. |
114 |
± |
10 |
|
| Wild type+K43E |
. |
. |
E |
. |
. |
. |
. |
90 |
± |
36 |
1× |
| M41L+T215Y |
. |
L |
. |
. |
. |
Y |
. |
1544 |
± |
402 |
14× |
| M41L+T215Y+E40F |
F |
L |
. |
. |
. |
Y |
. |
21307 |
± |
8810 |
187× |
| M41L+T215Y+K43E |
. |
L |
E |
. |
. |
Y |
. |
1556 |
± |
496 |
14× |
| M41L+T215Y+E40F+K43E |
F |
L |
E |
. |
. |
Y |
. |
15350 |
± |
5022 |
135× |
|
|
|||||||||||
| Pat A |
F |
L |
E |
V |
W |
Y |
T |
14739 |
± |
3105 |
129× |
| Pat A-WT40 |
. |
L |
E |
V |
W |
Y |
T |
1596 |
± |
377 |
14× |
| Pat A-WT43 |
F |
L |
. |
V |
W |
Y |
T |
13127 |
± |
4582 |
115× |
|
|
|||||||||||
|
Pat A-derived virus clone revealed additional amino acid changes including T200I, R211K, V245T, E248D, I293V and E297H The mean IC50 values of at least two experiments are shown (± standard error of the mean); in the most right column the fold increase compared to the wild type HIV-1 HXB2 reference strain is shown. |
|||||||||||
|
Huigen et al. Retrovirology 2008 5:20 doi:10.1186/1742-4690-5-20 |
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