Additional file 1.

Replication kinetics and Coreceptor usage of BL94 virus. (A) The ability of BL94 virus to propagate on PBMC. PBMC of donor BL94 were cocultured with CD8-depleted and mitogen-activated PBMC from a healthy donor. The production of p24 in the spent medium was monitored using a commercial antigen-capture ELISA kit. (B) Coreceptor phenotyping of the BL94 biological virus in HOS cell lines. HOS cell lines expressing CD4 and one of the several coreceptors including CCR1, CCR2B, CCR3, CXCR4 and CCR5 or CD4 alone or CCR5 alone were tested for viral infectivity. These cell lines were infected overnight with BL94 virus, residual virus was washed off and the cells were incubated up to 14 days. Secretion of p24 into the medium was monitored every third day using a commercial kit and data for day-7 are presented here. Data presented was representative of three independent experiments performed in triplicates.

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Dash et al. Retrovirology 2008 5:25   doi:10.1186/1742-4690-5-25