Table 1 |
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Primer sequences and PCR conditions |
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|
Target |
Primer |
Sequences (5'-3') |
PCR conditions |
|
|
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|
Gag |
For |
CCCATAGTGCAGAACATCCA |
50°C, 2 min, 95°C, 2 min, and 95°C, 15 s and 60°C, 30 s, for 50 cycles |
|
Rev |
GGGCTGAAAGCCTTCTCTTC |
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|
Singly spliced |
M669 |
GTGTGCCCGTCTGTTGTGTGACTCTGGTAAC |
50°C, 2 min, 95°C, 2 min, and 95°C, 15 s and 60°C, 30 s, for 50 cycles |
|
La 23 |
GCCTATTCTGCTATGTCGACACC |
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|
β -actin |
Actin F |
ATGGCCACGGCTGCTTCCAGC |
95°C, 15 s, 55°C, 30 s, 72°C, 15 s for 30 cycles |
|
Actin R |
CATGGTGGTGCCGCCAGACAG |
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|
GAPDH |
GAPDH F |
GAGTCAACGGATTTGGTCGT |
95°C, 15 s, 60°C, 30 s, 72°C, 15 s for 30 cycles |
|
GAPDH R |
TTGATTTTGGAGGGATCTCG |
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Giacaman et al. Retrovirology 2008 5:29 doi:10.1186/1742-4690-5-29 |
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