Figure 2.

Northern blot analysis of shRNA production in rtTA-shNef SupT1 cell lines. RNAs from the different HIV-rtTA-shNef SupT1 clones were isolated and separated on an agarose gel. After blotting the membrane was probed with a LNA probe against the shNef. Lane M, marker; lane 1, shNef clone B9; lane 2, clone C10 (a negative control cell line without a provirus); lane 3, shNef clone D2; lane 4, shNef clone D11; lane 5, shNef clone F7; lane 6, shNef clone F8; lane 7 and 8 contain positive controls from a transfection with the F-shNef^- and F-shNef⁺ plasmid [31]; lane 9 contains a negative empty-vector control; lane 10 in vitro produced shNef RNA.