Table 1

Overview of SupT1 clones with integrated HIV-rtTA provirus
Clone
 HIV-rtTA gDNA PCR
 HIV-1 resistant1
 Dox-induced
 Number detected proviruses







HIV-rtTA replication2
 Intracellular CA-p243




(24 h)
 (48 h)
 M.F.I.5
B5
 +
 -
 -
 0.01
 0.04
 -
 -4
B8
 +
 -
 -
 0.38
 0.34
 62
 -
C2
 +
 +
 -
 0.04
 0.00
 -
 1
C4
 +
 +
 -
 0.01
 0.00
 -
 -
C7
 +
 +
 -
 0.00
 0.00
 -
 2
D2
 +
 +
 -
 0.01
 0.06
 -
 1
D10
 +
 -
 -
 0.01
 0.02
 -
 -
D11
 +
 +
 -
 0.02
 0.01
 -
 1
E4
 +
 +
 -
 0.00
 0.01
 -
 2
F3
 +
 -
 -
 0.00
 0.01
 -
 -
H7
 +
 +
 -
 0.00
 0.00
 -
 3








B4
 +
 +
 +
 0.38
 0.50
 70
 1
B6
 +
 +
 +
 0.22
 0.19
 78
 5
B9
 +
 +
 +
 1.34
 1.66
 69
 3
C5
 +
 +
 +
 0.72
 0.82
 106
 3
C11
 +
 +
 +
 0.01
 0.04
 -
 3
D4
 +
 +
 +
 0.07
 0.02
 -
 4
D6
 +
 +
 +
 0.07
 0.02
 -
 1
E7
 +
 +
 +
 0.43
 0.59
 100
 -
E11
 +
 +
 +
 0.05
 0.02
 -
 -
F7
 +
 +
 +
 10.52
 11.54
 49
 2
F8
 +
 +
 +
 1.31
 2.16
 82
 1
F9
 +
 -
 +
 0.07
 0.02
 -
 1
G2
 +
 +
 +
 0.02
 0.01
 -
 1

1 Wild-type HIV-1 LAI superinfection

2 HIV-rtTA replication scored by syncytia formation and extracellular CA-p24 production

3 Percentage CA-p24 positive cells

4 -; not detected

5 M.F.I. is the mean fluorescence intensity of the CA-p24 positive cells (48 h dox)

Jeeninga et al. Retrovirology 2008 5:37   doi:10.1186/1742-4690-5-37