Table 1 |
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|
Detection of HTLV-2 sequences in PBMCs from inoculated rabbitsa |
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|
Weeks Post Inoculation |
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|
|
|||||
|
Inoculum and Rabbit |
0 |
2 |
6 |
8 |
11b |
|
|
|||||
|
729.HTLV-2 |
|||||
|
R27 |
- |
+ |
+ |
- |
+ (12.0) |
|
R28 |
- |
+ |
+ |
+ |
+ (8.3) |
|
R29 |
- |
+ |
+ |
+ |
+ (5.3) |
|
R30 |
- |
+ |
+ |
+ |
+ (32.8) |
|
R31 |
- |
+ |
+ |
- |
+ (10.7) |
|
R32 |
- |
+ |
+ |
+/- |
+ (4.2) |
|
729.HTLV-2Δp28 |
|||||
|
R20 |
- |
+ |
+/- |
+ |
- (0.2) |
|
R21 |
- |
+/- |
- |
- |
- (0.1) |
|
R22 |
- |
+/- |
- |
- |
- (0.3) |
|
R23 |
- |
+/- |
- |
- |
- (0.3) |
|
R24 |
- |
+/- |
- |
- |
- (1.1) |
|
R25 |
- |
- |
- |
- |
- (1.2) |
|
729 |
|||||
|
R1 |
- |
- |
- |
- |
- (0.1) |
|
R6 |
- |
- |
- |
- |
- (0.3) |
|
|
|||||
|
aGenomic DNA was isolated from rabbit PBMCs and subjected to standard PCR (40 cycles) using HTLV-2 specific primers (TRE-pH6-S/TRE-pH6-AS). -, no amplified PCR fragment; +, amplified PCR fragment. bNumbers in parentheses at wk 11 denote copy number per 1000 cells of rabbit PBMC as determined by real-time RT PCR. Copy numbers in rabbits inoculated with 729.HTLV-2Δp28 at wk 11 were significantly different than 729.HTLV-2 as determined by ANOVA followed by Turkey's test (p<0.00032) |
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|
Yamamoto et al. Retrovirology 2008 5:38 doi:10.1186/1742-4690-5-38 |
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