Figure 5.

In vitro binding interactions between MoMLV and HIV-1 integrases and selected proteins after treatment of the lysates with nucleases to eliminate nucleic acid bridges between the proteins. In vitro binding assays between the empty vector (MBP), full-length pmalc2-MoMLV IN (mIN) or full-length pmalc2-HIV-1 IN (hIN) and a subset of the clones isolated in the screen. All Westerns are loaded from left to right: MBP, mIN, hIN and the indicated GST fusion reactions. Upper panels, anti-MBP. Lower panels, anti-GST. (A) Left, maltose binding protein fusions with empty GST vector; right, MBP fusions with Ku70 and Brd2. (B) MBP fusions with U2AF²⁶, TFIIE-β, and Ankr49. (C) MBP fusions with the indicated proteins AF9, PRC, Fen-1, Baz2b, and Enx-1.