Figure 6.

Effect of 24 h-supernatants of blood T. cruzi trypomastigotes on cytokine/chemokine secretion in placental histocultures. Placental villi were transducted overnight with VSV-G pseudotyped HIV-1 (V) (100 ng p24/placental block) alone or in the presence of 24 h-supernatants of blood trypomastigotes (BTSn) (10⁶ parasites/placental block). Histoculture supernatants were collected after infection or coinfection, diluted with 10% FCS in RPMI and used for the simultaneous determination of cytokine/chemokine production with Luminex technology. Results displayed correspond to IL-6 (p < 0.04), IL-8 (p < 0.05), MIP-1α (p < 0.05), MIP-1β (p < 0.05), GM-CSF (p < 0.05) and MCP-1 (p < 0.01) (pg/ml). Results are expressed as the mean ± SD and are representative of 5 independent experiments.