Figure 8.

HIV-1 reverse transcription and nuclear import of the pre-integration complex are well supported in mouse T-cell lines after infection with nearly full-length HIV-1_NL4-3. (A) The relative levels of (upper panel) total HIV-1 cDNA, and (lower panel) 2-LTR circles were quantified in human (MT-4) and mouse (TIMI.4, R1.1) T-cell lines infected with VSV-G HIV-1_NL4-3 GFP on day 1 and 2 p.i., and samples derived from the experiment shown in Figs. 7A-C. At the indicated time points p.i. cell aliquots were taken and DNA analyzed by real-time PCR for the respective HIV-1 cDNA species. Duplicate samples were quantified. (B) Dynamic of HIV-1 cDNA load in passaged T-cell lines. Cells infected with VSV-G HIV-1_NL4-3 GFP BlaM-Vpr were cultivated for four weeks. The relative levels of total HIV-1 cDNA shown are derived from DNA analyses of two independent infections from cell samples taken on days 1, 14, and 28 p.i. *below detection limit.