Table 1

Effect of IFN-β treatment on retroviral restriction.

FOLD RESTRICTION

HIV-1

N-MLV

NB-MLV

B-MLV

SIVmac

MOI

0.5

5

0.5

5

0.5

5

0.5

5

0.5

5

MDTF

1.2

1.0

1.0

1.1

1.1

1.0

1.1

1.1

1.1

1.0

HeLa

1.5

1.1

3.7

7.6

1.9

1.4

nt

nt

nt

nt

CMMT

2.8

1.2

1.1

1.0

1.1

1.1

nt

nt

nt

nt

Vero

1.2

1.3

4.4

8.8

1.5

1.2

nt

nt

nt

nt

OMK

2.9

26.1

5.6

3.9

5.6

5.5

2.1

3.9

1.1

1.1

HeLa, CMMT, Vero or OMK cells were stimulated with IFN-β and transduced 24 h later at a MOI of 0.5 or 5 (as determined on MDTF cells) with HIV-1, N-MLV, NB-MLV, B-MLV or SIVmac, as indicated. The same experiment was performed in parallel on MDTF cells treated or not with universal type I IFN. Fold restriction represents the ratio of untreated to IFN-treated cells to be transduced by the GFP-encoding retroviral vectors. A significant enhancement of retroviral restriction is defined as a ratio > 2 (in bold). Data are from a typical experiment representative of three independent experiments. nt: not tested.

Carthagena et al. Retrovirology 2008 5:59   doi:10.1186/1742-4690-5-59